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blockpro tm blocking buffer  (Energenesis Biomedical)

 
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    Energenesis Biomedical blockpro tm blocking buffer
    Blockpro Tm Blocking Buffer, supplied by Energenesis Biomedical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blockpro+blocking+buffer/blocking+blockpro+buffer+tm/pmc13114095-95-13-17
    Average 86 stars, based on 1 article reviews
    blockpro tm blocking buffer - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Metformin induces apoptosis and inhibits migration by activating the AMPK/p53 axis and suppressing PI3K/AKT signaling in human cervical cancer cells
    Article Snippet: Proteins (30 μg) were incubated at 95°C for 10 min, separated via 10–12% SDS-PAGE and transferred to 0.2-μm PVDF membranes (Bio-Rad Laboratories, Inc.). .. Following blocking with BlockPRO blocking buffer (Energenesis Biomedical Co., Ltd.) for 1 h at room temperature, the membranes were incubated overnight at 4°C with the following primary antibodies (all 1:1,000): Monoclonal anti-p-AMPKα (Thr172; cat. no. 2535; Cell Signaling Technology, Inc.), monoclonal anti-AMPKα (cat. no. 5832; Cell Signaling Technology, Inc.), polyclonal anti-p-p53 (cat. no. 9284; Cell Signaling Technology, Inc.), polyclonal anti-p53 (cat. no. 9282; Cell Signaling Technology, Inc.), monoclonal anti-Bcl-2 (cat. no. 15071; Cell Signaling Technology, Inc.), monoclonal anti-Bax (cat. no. 5023; Cell Signaling Technology, Inc.), monoclonal anti-cleaved caspase-3 (cat. no. 9664; Cell Signaling Technology, Inc.), monoclonal anti-p-AKT (Ser473; cat. no. 4060; Cell Signaling Technology, Inc.), monoclonal anti-Akt (cat. no. 4298; Cell Signaling Technology, Inc.), monoclonal anti-p-p70S6 kinase (p70S6K; Thr389; cat. no. 9234; Cell Signaling Technology, Inc.), monoclonal anti-p70S6K (cat. no. 2708; Cell Signaling Technology, Inc.), polyclonal anti-Bcl-2 antagonist/killer 1 (Bak; cat. no. GTX100063; GeneTex, Inc.), polyclonal anti-phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α (PIK3CA; cat. no. NBP2-19804; Novus Biologicals, LLC) and monoclonal anti-GAPDH (cat. no. AC002; ABclonal Biotech Co., Ltd.). .. Following washing with TBST (Tris-buffered saline, 0.1% Tween-20), the membranes were incubated with an IgG HRP-conjugated secondary antibody (polyclonal anti-mouse; cat. no. 115-035-003; 1:50,000; Jackson ImmunoResearch Laboratories, Inc.; polyclonal anti-rabbit; cat. no. 31460; 1:100,000; Thermo Fisher Scientific Inc.) for 1 h at room temperature.

    Article Title: Anti-cancer activity and cellular uptake of 7,3′,4′- and 7,8,4′-trihydroxyisoflavone in HepG2 cells under hypoxic conditions
    Article Snippet: Proteins were separated using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). .. The membrane was blocked using BlockPRO Blocking Buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan) for 1 h at room temperature. ..

    Article Title: Metformin Potentiates the Anticancer Effect of Everolimus on Cervical Cancer In Vitro and In Vivo
    Article Snippet: Proteins (30 μg) were incubated at 95 °C for 10 min, separated on 10–12% ( w / v ) SDS-PAGE, and transferred onto 0.2-μm PVDF membranes (Bio-Rad, Irvine, CA, USA). .. Following blocking with a BlockPRO blocking buffer (Energenesis Biomedical) for 1 h at room temperature, membranes were probed with the following primary antibodies (1:1000 dilution) at 4 °C overnight: PARP (#9532), Bcl-2 (#15071), Bax (#5023), cleaved caspase-3 (#9664), P27Kip1 (#3686), phospho-AKT (Ser473) (#4060), Akt (#4298), phospho-P38 (#9211), P38 (#9212), phospho-ERK1/2 (#4370), ERK1/2 (#4695), phospho-JNK (#4668), JNK (#9258; Cell Signaling, Danvers, MA, USA); P21Cip1 (GTX629543), PCNA (GTX100539; GeneTex, Inc., Irvine, CA, USA); PIK3CA (NBP2-19804; Novus, CO, USA); MitoProfile ® Total OXPHOS rodent antibody cocktail (ab110413; Abcam, Cambridge, UK) and GAPDH (AC002, ABclonal Biotech, Woburn, MA, USA). .. Then, the membranes were washed and incubated with a secondary IgG-HRP-conjugated antibody (Mouse#115-035-003; Rabbit#111-035-003; Jackson ImmunoResearch, Laboratories, Inc., West Grove, PA, USA) for 1 h at room temperature.

    Article Title: Plasmon-activated water as a therapeutic strategy in Alzheimer’s disease by altering gut microbiota
    Article Snippet: After quantifying the protein content using the Bradford protein assay (Catalog number: 5000205; Bio-Rad, CA, USA), 20 μg of protein was separated using an 4-20 % sodium dodecylsulfate (SDS) polyacrylamide gradient gel (Catalog number: TFU-GG420; TOOLS, Taiwan), and then transferred to a polyvinylidene difluoride (PVDF) membrane (Catalog number: BSP0861; Pall, FL, USA). .. The membrane was blocked with BLOCKPRO blocking buffer (Catalog number: BM01-500; VISUAL PROTEIN, Taiwan) for 30 min, followed by incubation with a primary antibody at 4°C overnight. .. The antibody concentration of IL-1β (Catalog number: 12242; Cell Signaling, MA, USA), IL-6 (Catalog number: 12912; Cell Signaling), and TNF-α (Catalog number: 11498; Cell Signaling) used for western blotting were 2, 4, and 0.8 μg/mL.

    Article Title: Attenuation of Skeletal Muscle Atrophy Induced by Dexamethasone in Rats by Teaghrelin Supplementation
    Article Snippet: The protein samples were separated on 10% or 12.5% sodium dodecyl sulfate–polyacrylamide gels by electrophoresis and then transferred onto 0.22 μm polyvinylidene fluoride membranes (Merck Millipore). .. The membranes were soaked in BlockPRO blocking buffer (Energenesis Biomedical, Taipei, Taiwan) for 1 h at room temperature. .. They were then incubated with the following primary antibodies overnight at 4 °C: anti-AKT, -P-AKT, -FOXO1, -P-FOXO1, -MuRF1, -Atrogin-1, -LC3, -mTOR, -P-mTOR, -70S6K, -P-70S6K and -Glyceraldehyde 3-phosphate dehydrogenase (all purchased from Cell Signaling Technology, Boston, MA, USA).

    Article Title: Targeting lncRNA H19/miR-29b/COL1A1 Axis Impedes Myofibroblast Activities of Precancerous Oral Submucous Fibrosis.
    Article Snippet: After denaturation, equal amounts of extracted proteins (30 μg) were separated by 10% SDS-PAGE with Tris-Glycine SDS running buffer and transferred with transfer buffer onto a nitrocellulose membrane (GE 446 Healthcare, Little Chalfont, Buckinghamshire, UK). .. Then, transferred membranes were incubated with BlockPRO blocking buffer (visual protein, energenesis biomedical co. ltd). .. After washing with TBST buffer (20 mM Tris, 150 mM NaCl and 0.1% Tween 20; pH 7.4) for 5 min 3 times, the transferred membranes were incubated with the primary anti-bodies against α-SMA (1:1000 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), COL1A1(1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), fibronectin (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), or GADPH (1:5000 dilution; Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Plasmon-activated water as a therapeutic strategy in Alzheimer's disease by altering gut microbiota.
    Article Snippet: After quantifying the protein content using the Bradford protein assay (Catalog number: 5000205; Bio-Rad, CA, USA), 20 μg of protein was separated using an 4-20 % sodium dodecylsulfate (SDS) polyacrylamide gradient gel (Catalog number: TFUGG420; TOOLS, Taiwan), and then transferred to a polyvinylidene difluoride (PVDF) membrane (Catalog number: BSP0861; Pall, FL, USA). .. The membrane was blocked with BLOCKPRO blocking buffer (Catalog number: BM01-500; VISUAL PROTEIN, Taiwan) for 30 min, followed by incubation with a primary antibody at 4°C overnight. .. The antibody concentration of IL-1β (Catalog number: 12242; Cell Signaling, MA, USA), IL-6 (Catalog number: 12912; Cell Signaling), and TNF-α (Catalog number: 11498; Cell Signaling) used for western blotting were 2, 4, and 0.8 μg/mL.

    Incubation:

    Article Title: Metformin induces apoptosis and inhibits migration by activating the AMPK/p53 axis and suppressing PI3K/AKT signaling in human cervical cancer cells
    Article Snippet: Proteins (30 μg) were incubated at 95°C for 10 min, separated via 10–12% SDS-PAGE and transferred to 0.2-μm PVDF membranes (Bio-Rad Laboratories, Inc.). .. Following blocking with BlockPRO blocking buffer (Energenesis Biomedical Co., Ltd.) for 1 h at room temperature, the membranes were incubated overnight at 4°C with the following primary antibodies (all 1:1,000): Monoclonal anti-p-AMPKα (Thr172; cat. no. 2535; Cell Signaling Technology, Inc.), monoclonal anti-AMPKα (cat. no. 5832; Cell Signaling Technology, Inc.), polyclonal anti-p-p53 (cat. no. 9284; Cell Signaling Technology, Inc.), polyclonal anti-p53 (cat. no. 9282; Cell Signaling Technology, Inc.), monoclonal anti-Bcl-2 (cat. no. 15071; Cell Signaling Technology, Inc.), monoclonal anti-Bax (cat. no. 5023; Cell Signaling Technology, Inc.), monoclonal anti-cleaved caspase-3 (cat. no. 9664; Cell Signaling Technology, Inc.), monoclonal anti-p-AKT (Ser473; cat. no. 4060; Cell Signaling Technology, Inc.), monoclonal anti-Akt (cat. no. 4298; Cell Signaling Technology, Inc.), monoclonal anti-p-p70S6 kinase (p70S6K; Thr389; cat. no. 9234; Cell Signaling Technology, Inc.), monoclonal anti-p70S6K (cat. no. 2708; Cell Signaling Technology, Inc.), polyclonal anti-Bcl-2 antagonist/killer 1 (Bak; cat. no. GTX100063; GeneTex, Inc.), polyclonal anti-phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α (PIK3CA; cat. no. NBP2-19804; Novus Biologicals, LLC) and monoclonal anti-GAPDH (cat. no. AC002; ABclonal Biotech Co., Ltd.). .. Following washing with TBST (Tris-buffered saline, 0.1% Tween-20), the membranes were incubated with an IgG HRP-conjugated secondary antibody (polyclonal anti-mouse; cat. no. 115-035-003; 1:50,000; Jackson ImmunoResearch Laboratories, Inc.; polyclonal anti-rabbit; cat. no. 31460; 1:100,000; Thermo Fisher Scientific Inc.) for 1 h at room temperature.

    Article Title: Plasmon-activated water as a therapeutic strategy in Alzheimer’s disease by altering gut microbiota
    Article Snippet: After quantifying the protein content using the Bradford protein assay (Catalog number: 5000205; Bio-Rad, CA, USA), 20 μg of protein was separated using an 4-20 % sodium dodecylsulfate (SDS) polyacrylamide gradient gel (Catalog number: TFU-GG420; TOOLS, Taiwan), and then transferred to a polyvinylidene difluoride (PVDF) membrane (Catalog number: BSP0861; Pall, FL, USA). .. The membrane was blocked with BLOCKPRO blocking buffer (Catalog number: BM01-500; VISUAL PROTEIN, Taiwan) for 30 min, followed by incubation with a primary antibody at 4°C overnight. .. The antibody concentration of IL-1β (Catalog number: 12242; Cell Signaling, MA, USA), IL-6 (Catalog number: 12912; Cell Signaling), and TNF-α (Catalog number: 11498; Cell Signaling) used for western blotting were 2, 4, and 0.8 μg/mL.

    Article Title: Targeting lncRNA H19/miR-29b/COL1A1 Axis Impedes Myofibroblast Activities of Precancerous Oral Submucous Fibrosis.
    Article Snippet: After denaturation, equal amounts of extracted proteins (30 μg) were separated by 10% SDS-PAGE with Tris-Glycine SDS running buffer and transferred with transfer buffer onto a nitrocellulose membrane (GE 446 Healthcare, Little Chalfont, Buckinghamshire, UK). .. Then, transferred membranes were incubated with BlockPRO blocking buffer (visual protein, energenesis biomedical co. ltd). .. After washing with TBST buffer (20 mM Tris, 150 mM NaCl and 0.1% Tween 20; pH 7.4) for 5 min 3 times, the transferred membranes were incubated with the primary anti-bodies against α-SMA (1:1000 dilution; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), COL1A1(1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), fibronectin (1:1000 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA), or GADPH (1:5000 dilution; Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Plasmon-activated water as a therapeutic strategy in Alzheimer's disease by altering gut microbiota.
    Article Snippet: After quantifying the protein content using the Bradford protein assay (Catalog number: 5000205; Bio-Rad, CA, USA), 20 μg of protein was separated using an 4-20 % sodium dodecylsulfate (SDS) polyacrylamide gradient gel (Catalog number: TFUGG420; TOOLS, Taiwan), and then transferred to a polyvinylidene difluoride (PVDF) membrane (Catalog number: BSP0861; Pall, FL, USA). .. The membrane was blocked with BLOCKPRO blocking buffer (Catalog number: BM01-500; VISUAL PROTEIN, Taiwan) for 30 min, followed by incubation with a primary antibody at 4°C overnight. .. The antibody concentration of IL-1β (Catalog number: 12242; Cell Signaling, MA, USA), IL-6 (Catalog number: 12912; Cell Signaling), and TNF-α (Catalog number: 11498; Cell Signaling) used for western blotting were 2, 4, and 0.8 μg/mL.

    Membrane:

    Article Title: Anti-cancer activity and cellular uptake of 7,3′,4′- and 7,8,4′-trihydroxyisoflavone in HepG2 cells under hypoxic conditions
    Article Snippet: Proteins were separated using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). .. The membrane was blocked using BlockPRO Blocking Buffer (Energenesis Biomedical Co., Ltd., Taipei, Taiwan) for 1 h at room temperature. ..

    Article Title: Plasmon-activated water as a therapeutic strategy in Alzheimer’s disease by altering gut microbiota
    Article Snippet: After quantifying the protein content using the Bradford protein assay (Catalog number: 5000205; Bio-Rad, CA, USA), 20 μg of protein was separated using an 4-20 % sodium dodecylsulfate (SDS) polyacrylamide gradient gel (Catalog number: TFU-GG420; TOOLS, Taiwan), and then transferred to a polyvinylidene difluoride (PVDF) membrane (Catalog number: BSP0861; Pall, FL, USA). .. The membrane was blocked with BLOCKPRO blocking buffer (Catalog number: BM01-500; VISUAL PROTEIN, Taiwan) for 30 min, followed by incubation with a primary antibody at 4°C overnight. .. The antibody concentration of IL-1β (Catalog number: 12242; Cell Signaling, MA, USA), IL-6 (Catalog number: 12912; Cell Signaling), and TNF-α (Catalog number: 11498; Cell Signaling) used for western blotting were 2, 4, and 0.8 μg/mL.

    Article Title: Plasmon-activated water as a therapeutic strategy in Alzheimer's disease by altering gut microbiota.
    Article Snippet: After quantifying the protein content using the Bradford protein assay (Catalog number: 5000205; Bio-Rad, CA, USA), 20 μg of protein was separated using an 4-20 % sodium dodecylsulfate (SDS) polyacrylamide gradient gel (Catalog number: TFUGG420; TOOLS, Taiwan), and then transferred to a polyvinylidene difluoride (PVDF) membrane (Catalog number: BSP0861; Pall, FL, USA). .. The membrane was blocked with BLOCKPRO blocking buffer (Catalog number: BM01-500; VISUAL PROTEIN, Taiwan) for 30 min, followed by incubation with a primary antibody at 4°C overnight. .. The antibody concentration of IL-1β (Catalog number: 12242; Cell Signaling, MA, USA), IL-6 (Catalog number: 12912; Cell Signaling), and TNF-α (Catalog number: 11498; Cell Signaling) used for western blotting were 2, 4, and 0.8 μg/mL.



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